RT Journal Article
SR Electronic
T1 Rotten-skin disease significantly changed giant spiny frog(<em>Paa spinosa</em>) gut microbiota
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.13.905588
DO 10.1101/2020.01.13.905588
A1 Tuoyu He
A1 Yun Jiang
A1 Pengpeng Wang
A1 Jianguo Xiang
A1 Wangcheng Pan
YR 2020
UL http://biorxiv.org/content/early/2020/01/14/2020.01.13.905588.abstract
AB The composition and abundance of gut microbiota is essential for host health and immunity. Gut microbiota is symbiotic with the host, so changes in the host diet, development, and health will lead to changes in the gut microbiota. Conversely, changes in the gut microbiota also affect the host conditions. In this experiment, 16S rRNA high-throughput sequencing was used to compare the gut microbiota composition of 5 healthy Paa Spinosa and 6 P. spinosa with rotten-skin disease. Results: the gut microbiota composition was significant difference between diseased P. spinosa and the healthy P. spinosa; LEfSe analysis showed that the relative abundance of Methanocorpusculum, Parabacteroides, AF12, PW3, Epulopiscium, and Oscillospira were significantly higher in the diseased P. spinosa, while the relative abundance of Serratia, Eubacteium, Citrobacter, and Morganella were significantly lower. Conclusion: Rotten-skin disease changed P. spinosa gut microbiota significantly; The relative abundance of Epulopiscium and Oscillospira might be related to the health conditions of the host skin and gallbladder; The relative abundance of Serratia and Eubacteium might be important for maintaining the gut microbiota ecosystem.