PT  - JOURNAL ARTICLE
AU  - Claire A. de March
AU  - William B. Titlow
AU  - Tomoko Sengoku
AU  - Patrick Breheny
AU  - Hiroaki Matsunami
AU  - Timothy S. McClintock
TI  - Modulation of the combinatorial code of odorant receptor response patterns in odorant mixtures
AID  - 10.1101/865386
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 865386
4099  - http://biorxiv.org/content/early/2020/01/15/865386.short
4100  - http://biorxiv.org/content/early/2020/01/15/865386.full
AB  - The perception of odors relies on combinatorial codes consisting of odorant receptor (OR) response patterns to encode odor identity. The modulation of these patterns by odorant interactions at ORs potentially explains several olfactory phenomena: mixture suppression, unpredictable sensory outcomes, and the perception of odorant mixtures as unique objects. We determined OR response patterns to 4 odorants and 3 binary mixtures in vivo in mice, identifying 30 responsive ORs. These patterns typically had a few strongly responsive ORs and a greater number of weakly responsive ORs. The ORs responsive to an odorant were often unrelated sequences distributed across several OR subfamilies. Mixture responses predicted pharmacological interactions between odorants, which were tested in vitro by heterologous expression of ORs in cultured cells. These tests provided independent evidence confirming odorant agonists for 13 ORs and identified both suppressive and additive effects of mixing odorants. This included 11 instances of antagonism of ORs by an odorant, 1 instance of additive responses to a binary mixture, 1 instance of suppression of a strong agonist by a weak agonist, and the discovery of an inverse agonist for an OR. These findings demonstrate that interactions between odorants at ORs are common.