PT  - JOURNAL ARTICLE
AU  - Benedetta Artegiani
AU  - Delilah Hendriks
AU  - Joep Beumer
AU  - Rutger Kok
AU  - Xuan Zheng
AU  - Indi Joore
AU  - Susana Chuva de Sousa Lopes
AU  - Jeroen van Zon
AU  - Sander Tans
AU  - Hans Clevers
TI  - Fast and efficient generation of knock-in human organoids using homology-independent CRISPR/Cas9 precision genome editing
AID  - 10.1101/2020.01.15.907766
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.01.15.907766
4099  - http://biorxiv.org/content/early/2020/01/15/2020.01.15.907766.short
4100  - http://biorxiv.org/content/early/2020/01/15/2020.01.15.907766.full
AB  - CRISPR/Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences in human organoids awaits robust knock-in approaches. Here, we describe CRISPR/Cas9-mediated Homology-independent Organoid Transgenesis (CRISPR-HOT), which allows efficient generation of knock-in human organoids representing different tissues. CRISPR-HOT avoids extensive cloning and outperforms homology directed repair (HDR) in achieving precise integration of exogenous DNA sequences at desired loci, without the necessity to inactivate TP53 in untransformed cells, previously used to increase HDR-mediated knock-in. CRISPR-HOT was employed to fluorescently tag and visualize subcellular structural molecules and to generate reporter lines for rare intestinal cell types. A double reporter labelling the mitotic spindle by tagged tubulin and the cell membrane by tagged E-cadherin uncovered modes of human hepatocyte division. Combining tubulin tagging with TP53 knock-out revealed TP53 involvement in controlling hepatocyte ploidy and mitotic spindle fidelity. CRISPR-HOT simplifies genome editing in human organoids.