PT - JOURNAL ARTICLE AU - The International Brain Laboratory AU - Valeria Aguillon-Rodriguez AU - Dora E. Angelaki AU - Hannah M. Bayer AU - Niccolò Bonacchi AU - Matteo Carandini AU - Fanny Cazettes AU - Gaelle A. Chapuis AU - Anne K. Churchland AU - Yang Dan AU - Eric E. Dewitt AU - Mayo Faulkner AU - Hamish Forrest AU - Laura M. Haetzel AU - Michael Hausser AU - Sonja B. Hofer AU - Fei Hu AU - Anup Khanal AU - Christopher S. Krasniak AU - Inês Laranjeira AU - Zachary F. Mainen AU - Guido T. Meijer AU - Nathaniel J. Miska AU - Thomas D. Mrsic-Flogel AU - Masayoshi Murakami AU - Jean-Paul Noel AU - Alejandro Pan-Vazquez AU - Josh I. Sanders AU - Karolina Z. Socha AU - Rebecca Terry AU - Anne E. Urai AU - Hernando M. Vergara AU - Miles J. Wells AU - Christian J. Wilson AU - Ilana B. Witten AU - Lauren E. Wool AU - Anthony Zador TI - A standardized and reproducible method to measure decision-making in mice AID - 10.1101/2020.01.17.909838 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.01.17.909838 4099 - http://biorxiv.org/content/early/2020/01/19/2020.01.17.909838.short 4100 - http://biorxiv.org/content/early/2020/01/19/2020.01.17.909838.full AB - Progress in neuroscience is hindered by poor reproducibility of mouse behavior. Here we show that in a visual decision making task, reproducibility can be achieved by automating the training protocol and by standardizing experimental hardware, software, and procedures. We trained 101 mice in this task across seven laboratories at six different research institutions in three countries, and obtained 3 million mouse choices. In trained mice, variability in behavior between labs was indistinguishable from variability within labs. Psychometric curves showed no significant differences in visual threshold, bias, or lapse rates across labs. Moreover, mice across laboratories adopted similar strategies when stimulus location had asymmetrical probability that changed over time. We provide detailed instructions and open-source tools to set up and implement our method in other laboratories. These results establish a new standard for reproducibility of rodent behavior and provide accessible tools for the study of decision making in mice.