RT Journal Article
SR Electronic
T1 Hydrogen sulfide promotes nodulation and nitrogen fixation in soybean-rhizobia symbiotic system
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 367896
DO 10.1101/367896
A1 Hang Zou
A1 Ni-Na Zhang
A1 Qing Pan
A1 Jian-Hua Zhang
A1 Juan Chen
A1 Ge-Hong Wei
YR 2018
UL http://biorxiv.org/content/early/2018/07/12/367896.abstract
AB The rhizobium-legume symbiotic system is crucial for nitrogen cycle balance in agriculture. Hydrogen sulfide (H2S), a gaseous signaling molecule, may regulate various physiological processes in plants. However, whether H2S has regulatory effect in this symbiotic system remains unknown. Herein, we investigated the possible role of H2S in the symbiosis between soybean (Glycine max) and rhizobium (Sinorhizobium fredii). Our results demonstrated that exogenous H2S donor (sodium hydrosulfide, NaHS) treatment promoted soybean growth, nodulation and nitrogenase (Nase) activity. Western blotting analysis revealed that the abundance of nitrogenase component nifH was increased by NaHS treatment in nodules. Quantitative real-time PCR data showed that NaHS treatment up-regulated the expressions of symbiosis-related genes nodC and nodD of S. fredii. Besides, expression of soybean nodulation marker genes including early nodulin 40 (GmENOD40), ERF required for nodulation (GmERN), nodulation signaling pathway2b (GmNSP2b) and nodulation inception genes (GmNIN1a, GmNIN2a and GmNIN2b) were up-regulated. Moreover, the expressions of glutamate synthase (GmGS), nitrite reductase (GmNiR), ammonia transporter (GmSAT1), and nifH involved in nitrogen metabolism were up-regulated in NaHS-treated soybean roots and nodules. Together, our results suggested that H2S may act as a positive signaling molecule in soybean-rhizobia symbiotic system and enhance their nitrogen fixation ability.Highlight We demonstrated for the first time that H2S as a signaling molecule may promote the establishment of symbiotic relationship and nitrogen fixation ability in the soybean-rhizobia symbiotic system.AbbreviationsCicarbon dioxide concentration.Fmmaximal fluorescence yield.Fominimal fluorescence yield.Gsstomatal conductance.Pnphotosynthetic rate.PSIIphotosystem II.qRT-PCRquantitative real-time PCR.Trtranspiration rate.Vpdlvapor pressure divicit.