RT Journal Article
SR Electronic
T1 Chimera-competent eXtra-Embryonic eNdoderm (XEN) cells established from pig embryos
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.02.892802
DO 10.1101/2020.01.02.892802
A1 Chi Park
A1 Young Jeoung
A1 Jun Uh
A1 Kieun Park
A1 Jessica Bridge
A1 Anne Powell
A1 Jie Li
A1 Laramie Pence
A1 Tianbin Liu
A1 Hai-Xi Sun
A1 Ying Gu
A1 Yue Shen
A1 Jun Wu
A1 Juan-Carlos Izpisua Belmonte
A1 Bhanu P. Telugu
YR 2020
UL http://biorxiv.org/content/early/2020/01/23/2020.01.02.892802.abstract
AB In this article, we report for the first time the derivation and characterization of extra-embryonic endoderm (XEN) cells from primitive endoderm (PrE) of porcine (p) embryos. The pXEN cells can be reliably and reproducibly generated from parthenote, in vitro and in vivo derived embryos. The pXEN cells retained all the hallmarks of PrE including expression of canonical PrE and XEN cell markers (GATA4, GATA6, SOX17, SALL4, FOXA2, and HNF4A). Transcriptome analysis further confirmed their XEN cell origin. The pXEN cells when introduced into blastocyst stage embryo contributed to wide-spread chimerism including visceral yolk sac, chorion, as well as embryonic gut and liver primordium in the fetus. The pXEN cells were shown to be an efficient nuclear donor for generating cloned offspring. Taken together, pXEN cells fulfil a longstanding need for a stable, chimera-competent, and nuclear transfer-compatible porcine embryonic cells with applications for agriculture and medicine.Significance Statement We report for the first time, the derivation and characterization of extraembryonic endoderm (XEN) stem cells from porcine (p) embryos. The pXEN cells can be reliably and reproducibly derived from primitive endoderm precursors. When injected into blastocyst-stage embryos, the pXEN cells have contributed to wide-spread chimerism including visceral yolk sac, chorion of the extraembryonic membranes, as well as definitive endoderm of the fetus, primarily the embryonic gut and liver primordium. Additionally, these XEN cells have proven to be an efficient nuclear donor for generating cloned offspring. These newly discovered stem cells provide a novel model for studying lineage segregation, as well as a source for interspecies chimeras for generating endodermal organs, and for genome editing in livestock.