TY - JOUR T1 - Brd4 and P300 regulate zygotic genome activation through histone acetylation JF - bioRxiv DO - 10.1101/369231 SP - 369231 AU - Shun Hang Chan AU - Yin Tang AU - Liyun Miao AU - Hiba Darwich-Codore AU - Charles E. Vejnar AU - Jean-Denis Beaudoin AU - Damir Musaev AU - Juan P. Fernandez AU - Miguel A. Moreno-Mateos AU - Antonio J. Giraldez Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/07/13/369231.abstract N2 - The awakening of the zygote genome, signaling the transition from maternal transcriptional control to zygotic control, is a watershed in embryonic development, but the factors and mechanisms controlling this transition are still poorly understood. By combining CRISPR-Cas9-mediated live imaging of the first transcribed genes (miR-430), chromatin and transcription analysis during zebrafish embryogenesis, we observed that genome activation is gradual and stochastic, and the active state is inherited in daughter cells. We discovered that genome activation is regulated through both translation of maternal mRNAs and the effects of these factors on the chromatin. We show that chemical inhibition of H3K27Ac writer (P300) and reader (Brd4) block genome activation, while induction of a histone acetylation prematurely activates transcription, and restore genome activation in embryos where translation of maternal mRNAs is impaired, demonstrating that they are limiting factors for the activation of the genome. In contrast to current models, we do not observe triggering of genome activation by a reduction of the nuclear-cytoplasmic (N/C) ratio or slower cell division. We conclude that genome activation is controlled by a time-dependent mechanism involving the translation of maternal mRNAs and the regulation of histone acetylation through P300 and Brd4. This mechanism is critical to initiating zygotic development and developmental reprogramming. ER -