%0 Journal Article %A Vera D. Rinaldi %A Elisa Donnard %A Kyle J. Gellatly %A Morten Rasmussen %A Alper Kucukural %A Onur Yukselen %A Manuel Garber %A Upasna Sharma %A Oliver J. Rando %T An atlas of cell types in the mammalian epididymis and vas deferens %D 2020 %R 10.1101/2020.01.24.918979 %J bioRxiv %P 2020.01.24.918979 %X Following spermatogenesis in the testis, mammalian sperm continue to mature over the course of approximately 10 days as they transit a long epithelial tube known as the epididymis. The epididymis is comprised of multiple segments/compartments that, in addition to concentrating sperm and preventing their premature activation, play key roles in remodeling the protein, lipid, and RNA composition of maturing sperm. In order to understand the complex roles for the epididymis in reproductive biology, we generated a single cell atlas of gene expression from the murine epididymis and vas deferens. We recovered all the key cell types of the epididymal epithelium, including principal cells, clear cells, and basal cells, along with associated support cells that include fibroblasts, smooth muscle, macrophages and other immune cells. Moreover, our data illuminate extensive regional specialization of principal cell populations across the length of the epididymis, with a substantial fraction of segment-specific genes localized in genomic clusters of functionally-related genes. In addition to the extensive region-specific specialization of principal cells, we find evidence for functionally-specialized subpopulations of stromal cells, and, most notably, two distinct populations of clear cells. Analysis of ligand/receptor expression reveals a network of potential cellular signaling connections, with several predicted interactions between cell types that may play roles in immune cell recruitment and other aspects of epididymal function. Our dataset extends on existing knowledge of epididymal biology, and provides a wealth of information on potential regulatory and signaling factors that bear future investigation. %U https://www.biorxiv.org/content/biorxiv/early/2020/01/24/2020.01.24.918979.full.pdf