RT Journal Article
SR Electronic
T1 Proteomic changes in bacteria caused by exposure to environmental conditions can be detected by Matrix-Assisted Laser Desorption/Ionization – Time of Flight (MALDI-ToF) Mass Spectrometry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.24.918938
DO 10.1101/2020.01.24.918938
A1 Denise Chac
A1 Melissa Kordahi
A1 Leandra Brettner
A1 Arushi Verma
A1 Paul McCleary
A1 Kelly Crebs
A1 Cara Yee
A1 R. William DePaolo
YR 2020
UL http://biorxiv.org/content/early/2020/01/25/2020.01.24.918938.abstract
AB In the past decade, matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry (MS) has become a timely and cost-effective alternative to bacterial identification. The MALDI-ToF MS technique analyzes the total protein of culturable microorganisms at the species level and produces a mass spectra based on peptides which is compared to a database of identified profiles. Consequently, unique signatures of each microorganism are produced allowing identification at the species and, more importantly, strain level. Our present study proposes that the MALDI-ToF MS can be further used to screen functional and metabolic differences. While other studies applied the MALDI-ToF technique to identify subgroups within species, we investigated how various environmental factors could alter the unique bacterial signatures. We found that genetic and phenotypic differences between microorganisms belonging to the same species can be reflected in peptide-mass fingerprints generated by MALDI-ToF MS. These results suggest that MALDI-ToF MS can screen intra-species phenotypic differences of several microorganisms.