RT Journal Article
SR Electronic
T1 Cell-type specific D1 dopamine receptor modulation of projection neurons and interneurons in the prefrontal cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 370536
DO 10.1101/370536
A1 Paul G. Anastasiades
A1 Christina Boada
A1 Adam G. Carter
YR 2018
UL http://biorxiv.org/content/early/2018/07/16/370536.abstract
AB Dopamine modulation in the prefrontal cortex (PFC) mediates diverse effects on neuronal physiology and function, but the expression of dopamine receptors at sub-populations of pyramidal neurons and interneurons remains unresolved. Here, we examine D1 receptor expression and modulation at specific cell types and layers in the mouse prelimbic PFC. We first show that D1 receptors are enriched in pyramidal neurons in both layers 5 and 6, and that these cells project intra-telencephalically, rather than to sub-cortical structures. We then find that D1 receptors are also present in interneurons, and enriched in VIP+ interneurons that co-expresses calretinin, but absent from PV+ and SOM+ interneurons. Finally, we determine that D1 receptors strongly and selectively enhance action potential firing in only a subset of these cortico-cortical neurons and VIP+ interneurons. Our findings define several novel sub-populations of D1+ neurons, highlighting how modulation via D1 receptors can influence both excitatory and disinhibitory micro-circuits in the PFC.