RT Journal Article
SR Electronic
T1 Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.27.918193
DO 10.1101/2020.01.27.918193
A1 Lindsey Barske
A1 Peter Fabian
A1 Christine Hirschberger
A1 David Jandzik
A1 Tyler Square
A1 Pengfei Xu
A1 Nellie Nelson
A1 Haoze Vincent Yu
A1 Daniel M. Medeiros
A1 J. Andrew Gillis
A1 J. Gage Crump
YR 2020
UL http://biorxiv.org/content/early/2020/01/28/2020.01.27.918193.abstract
AB Whereas the gill chambers of extant jawless vertebrates (lampreys and hagfish) open directly into the environment, jawed vertebrates evolved skeletal appendages that promote the unidirectional flow of oxygenated water over the gills. A major anatomical difference between the two jawed vertebrate lineages is the presence of a single large gill cover in bony fishes versus separate covers for each gill chamber in cartilaginous fishes. Here we find that these divergent gill cover patterns correlate with the pharyngeal arch expression of Pou3f3 orthologs. We identify a Pou3f3 arch enhancer that is deeply conserved from cartilaginous fish through humans but undetectable in lampreys, with minor sequence differences in the bony versus cartilaginous fish enhancers driving the corresponding single versus multiple gill arch expression patterns. In zebrafish, loss of Pou3f3 gene function disrupts gill cover formation, and forced expression of Pou3f3b in the gill arches generates ectopic skeletal elements resembling the multiple gill cover pattern of cartilaginous fishes. Emergence of this Pou3f3 enhancer >430 mya and subsequent modifications may thus have contributed to the acquisition and diversification of gill covers and respiratory strategies during gnathostome evolution.