RT Journal Article
SR Electronic
T1 Quantitative comparison of single-cell sequencing methods using hippocampal neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 004366
DO 10.1101/004366
A1 Luwen Ning
A1 Guan Wang
A1 Zhoufang Li
A1 Wen Hu
A1 Qingming Hou
A1 Yin Tong
A1 Meng Zhang
A1 Li Qin
A1 Xiaoping Chen
A1 Hengye Man
A1 Pinghua Liu
A1 Jiankui He
YR 2014
UL http://biorxiv.org/content/early/2014/04/21/004366.abstract
AB Single-cell genomic analysis has grown rapidly in recent years and will find widespread applications in various fields of biology, including cancer biology, development, immunology, pre-implantation genetic diagnosis, and neurobiology. In this study, we amplified genomic DNA from individual hippocampal neurons using one of three single-cell DNA amplification methods (multiple annealing and looping-based amplification cycles (MALBAC), multiple displacement amplification (MDA), and GenomePlex whole genome amplification (WGA4)). We then systematically evaluated the genome coverage, GC-bias, reproducibility, and copy number variations among individual neurons. Our results showed that single-cell genome sequencing results obtained from the MALBAC and WGA4 methods are highly reproducible and have a high success rate. Chromosome-level and subchromosomal-level copy number variations among individual neurons can be detected.