TY - JOUR T1 - A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe <em>Pet1</em> neurons JF - bioRxiv DO - 10.1101/2020.01.28.923375 SP - 2020.01.28.923375 AU - Benjamin W. Okaty AU - Nikita Sturrock AU - Yasmin Escobedo Lozoya AU - YoonJeung Chang AU - Krissy A. Lyon AU - Olga V. Alekseyenko AU - Susan M. Dymecki Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/01/29/2020.01.28.923375.abstract N2 - The dorsal raphe nucleus (DR) contains the largest brain population of Pet1-lineage neurons, a predominantly serotonergic group distributed throughout multiple DR subdomains. These neurons collectively regulate diverse physiology and behavior and are often therapeutically targeted to treat affective disorders. Characterizing Pet1 neuron molecular heterogeneity and relating it to anatomy is vital for understanding DR functional organization, with potential to inform therapeutic separability. Here we use high-throughput and DR subdomain-targeted single-cell transcriptomics and intersectional genetic tools to map molecular and anatomical diversity of DR-Pet1 neurons. We describe up to fourteen neuron subtypes, many showing biased cell body distributions across the DR. We further show that P2ry1-Pet1 DR neurons – the most molecularly distinct of the subtypes – possess unique efferent projections and electrophysiological properties. The present data complement and extend previous DR characterizations, combining intersectional genetics with multiple transcriptomic modalities to achieve fine-scale molecular and anatomic identification of Pet1 neuron subtypes. ER -