PT - JOURNAL ARTICLE AU - Clark, Matt Q. AU - McCumsey, Stephanie J. AU - Lopez-Darwin, Sereno AU - Heckscher, Ellie S. AU - Doe, Chris Q. TI - Functional Genetic Screen to Identify Interneurons Governing Behaviorally Distinct Aspects of <em>Drosophila</em> Larval Motor Programs AID - 10.1101/041061 DP - 2016 Jan 01 TA - bioRxiv PG - 041061 4099 - http://biorxiv.org/content/early/2016/02/28/041061.short 4100 - http://biorxiv.org/content/early/2016/02/28/041061.full AB - Drosophila larval crawling is an attractive system to study patterned motor output at the level of animal behavior. Larval crawling consists of waves of muscle contractions generating forward or reverse locomotion. In addition, larvae undergo additional behaviors including head casts, turning, and feeding. It is likely that some neurons are used in all these behaviors (e.g. motor neurons), but the identity (or even existence) of neurons dedicated to specific aspects of behavior is unclear. To identify neurons that regulate specific aspects of larval locomotion, we performed a genetic screen to identify neurons that, when activated, could elicit distinct motor programs. We used 165 Janelia CRM-Gal4 lines – chosen for sparse neuronal expression – to express the warmth-inducible neuronal activator TrpA1 and screened for locomotor defects. The primary screen measured forward locomotion velocity, and we identified 63 lines that had locomotion velocities significantly slower than controls following TrpA1 activation (28°C). A secondary screen was performed on these lines, revealing multiple discrete behavioral phenotypes including slow forward locomotion, excessive reverse locomotion, excessive turning, excessive feeding, immobile, rigid paralysis, and delayed paralysis. While many of the Gal4 lines had motor, sensory, or muscle expression that may account for some or all of the phenotype, some lines showed specific expression in a sparse pattern of interneurons. Our results show that distinct motor programs utilize distinct subsets of interneurons, and provide an entry point for characterizing interneurons governing different elements of the larval motor program.