PT  - JOURNAL ARTICLE
AU  - Laleh Haghverdi
AU  - Maren Büttner
AU  - F. Alexander Wolf
AU  - Florian Buettner
AU  - Fabian J. Theis
TI  - Diffusion pseudonyme robustly reconstructs lineage branching
AID  - 10.1101/041384
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 041384
4099  - http://biorxiv.org/content/early/2016/02/29/041384.short
4100  - http://biorxiv.org/content/early/2016/02/29/041384.full
AB  - Single-cell gene expression profiles of differentiating cells encode their intrinsic latent temporal order. We describe an efficient way to robustly estimate this order according to a diffusion pseudotime, which measures transitions on all length scales between cells using diffusion-like random walks. This allows us to identify cells that undergo branching decisions or are in metastable states, and thereby genes differentially regulated at these states.