RT Journal Article
SR Electronic
T1 Complete genome characterisation of a novel coronavirus associated with severe human respiratory disease in Wuhan, China
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.24.919183
DO 10.1101/2020.01.24.919183
A1 Fan Wu
A1 Su Zhao
A1 Bin Yu
A1 Yan-Mei Chen
A1 Wen Wang
A1 Yi Hu
A1 Zhi-Gang Song
A1 Zhao-Wu Tao
A1 Jun-Hua Tian
A1 Yuan-Yuan Pei
A1 Ming-Li Yuan
A1 Yu-Ling Zhang
A1 Fa-Hui Dai
A1 Yi Liu
A1 Qi-Min Wang
A1 Jiao-Jiao Zheng
A1 Lin Xu
A1 Edward C. Holmes
A1 Yong-Zhen Zhang
YR 2020
UL http://biorxiv.org/content/early/2020/02/02/2020.01.24.919183.abstract
AB Emerging and re-emerging infectious diseases, such as SARS, MERS, Zika and highly pathogenic influenza present a major threat to public health1–3. Despite intense research effort, how, when and where novel diseases appear are still the source of considerable uncertainly. A severe respiratory disease was recently reported in the city of Wuhan, Hubei province, China. At the time of writing, at least 62 suspected cases have been reported since the first patient was hospitalized on December 12nd 2019. Epidemiological investigation by the local Center for Disease Control and Prevention (CDC) suggested that the outbreak was associated with a sea food market in Wuhan. We studied seven patients who were workers at the market, and collected bronchoalveolar lavage fluid (BALF) from one patient who exhibited a severe respiratory syndrome including fever, dizziness and cough, and who was admitted to Wuhan Central Hospital on December 26th 2019. Next generation metagenomic RNA sequencing4 identified a novel RNA virus from the family Coronaviridae designed WH-Human-1 coronavirus (WHCV).Phylogenetic analysis of the complete viral genome (29,903 nucleotides) revealed that WHCV was most closely related (89.1% nucleotide similarity similarity) to a group of Severe Acute Respiratory Syndrome (SARS)-like coronaviruses (genus Betacoronavirus, subgenus Sarbecovirus) previously sampled from bats in China and that have a history of genomic recombination. This outbreak highlights the ongoing capacity of viral spill-over from animals to cause severe disease in humans.