TY - JOUR T1 - Quantitative, real-time, single cell analysis in tissue reveals expression dynamics of neurogenesis JF - bioRxiv DO - 10.1101/373407 SP - 373407 AU - Cerys S Manning AU - Veronica Biga AU - James Boyd AU - Jochen Kursawe AU - Bodvar Ymisson AU - David G Spiller AU - Christopher M Sanderson AU - Tobias Galla AU - Magnus Rattray AU - Nancy Papalopulu Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/07/20/373407.abstract N2 - Despite single cell transcriptomics, how cells make transitions within tissues in real time, is not understood. Here, we use single cell live imaging of an endogenous HES5 reporter and absolute quantification to gain a dynamic view of neurogenesis in the embryonic mammalian spinal cord. We show that HES5 protein expression fluctuates in dividing neural progenitors and becomes more frequently periodic in the transition towards differentiation, creating transient oscillations with higher fold-changes. This dynamic behavior means that the HES5 population heterogeneity observed between cells at a fixed time-point, is a composite of short-term and longer-term dynamics. ER -