PT  - JOURNAL ARTICLE
AU  - Ruiyao Cai
AU  - Chenchen Pan
AU  - Alireza Ghasemigharagoz
AU  - Mihail I. Todorov
AU  - Benjamin Förstera
AU  - Shan Zhao
AU  - Harsharan S. Bhatia
AU  - Leander Mrowka
AU  - Delphine Theodorou
AU  - Markus Rempfler
AU  - Anna Xavier
AU  - Benjamin T. Kress
AU  - Corinne Benakis
AU  - Arthur Liesz
AU  - Bjoern Menze
AU  - Martin Kerschensteiner
AU  - Maiken Nedergaard
AU  - Ali Ertürk
TI  - Panoptic vDISCO imaging reveals neuronal connectivity, remote trauma effects and meningeal vessels in intact transparent mice
AID  - 10.1101/374785
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 374785
4099  - http://biorxiv.org/content/early/2018/07/23/374785.short
4100  - http://biorxiv.org/content/early/2018/07/23/374785.full
AB  - Analysis of entire transparent rodent bodies could provide holistic information on biological systems in health and disease. However, it has been challenging to reliably image and quantify signal from endogenously expressed fluorescent proteins in large cleared mouse bodies due to the low signal contrast. Here, we devised a pressure driven, nanobody based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image subcellular details in transparent mouse bodies through bones and highly autofluorescent tissues, and perform quantifications. We visualized for the first-time whole-body neuronal connectivity of an entire adult mouse and discovered that brain trauma induces degeneration of peripheral axons. We also imaged meningeal lymphatic vessels and immune cells through the intact skull and vertebra in naïve animals and trauma models. Thus, our new approach can provide an unbiased holistic view of biological events affecting the nervous system and the rest of the body.Note: Manuscript videos are available at ‘Supplementary material’ section of BioRxiv and at the following link http://vdisco.isd-muc.de/