RT Journal Article
SR Electronic
T1 Panoptic vDISCO imaging reveals neuronal connectivity, remote trauma effects and meningeal vessels in intact transparent mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 374785
DO 10.1101/374785
A1 Ruiyao Cai
A1 Chenchen Pan
A1 Alireza Ghasemigharagoz
A1 Mihail I. Todorov
A1 Benjamin Förstera
A1 Shan Zhao
A1 Harsharan S. Bhatia
A1 Leander Mrowka
A1 Delphine Theodorou
A1 Markus Rempfler
A1 Anna Xavier
A1 Benjamin T. Kress
A1 Corinne Benakis
A1 Arthur Liesz
A1 Bjoern Menze
A1 Martin Kerschensteiner
A1 Maiken Nedergaard
A1 Ali Ertürk
YR 2018
UL http://biorxiv.org/content/early/2018/07/23/374785.abstract
AB Analysis of entire transparent rodent bodies could provide holistic information on biological systems in health and disease. However, it has been challenging to reliably image and quantify signal from endogenously expressed fluorescent proteins in large cleared mouse bodies due to the low signal contrast. Here, we devised a pressure driven, nanobody based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image subcellular details in transparent mouse bodies through bones and highly autofluorescent tissues, and perform quantifications. We visualized for the first-time whole-body neuronal connectivity of an entire adult mouse and discovered that brain trauma induces degeneration of peripheral axons. We also imaged meningeal lymphatic vessels and immune cells through the intact skull and vertebra in naïve animals and trauma models. Thus, our new approach can provide an unbiased holistic view of biological events affecting the nervous system and the rest of the body.Note: Manuscript videos are available at ‘Supplementary material’ section of BioRxiv and at the following link http://vdisco.isd-muc.de/