RT Journal Article
SR Electronic
T1 Toward an integrated classification of neuronal cell types: morphoelectric and transcriptomic characterization of individual GABAergic cortical neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.03.932244
DO 10.1101/2020.02.03.932244
A1 Nathan W. Gouwens
A1 Staci A. Sorensen
A1 Fahimeh Baftizadeh
A1 Agata Budzillo
A1 Brian R. Lee
A1 Tim Jarsky
A1 Lauren Alfiler
A1 Anton Arkhipov
A1 Katherine Baker
A1 Eliza Barkan
A1 Kyla Berry
A1 Darren Bertagnolli
A1 Kris Bickley
A1 Jasmine Bomben
A1 Thomas Braun
A1 Krissy Brouner
A1 Tamara Casper
A1 Kirsten Crichton
A1 Tanya L. Daigle
A1 Rachel Dalley
A1 Rebecca de Frates
A1 Nick Dee
A1 Tsega Desta
A1 Samuel Dingman Lee
A1 Nadezhda Dotson
A1 Tom Egdorf
A1 Lauren Ellingwood
A1 Rachel Enstrom
A1 Luke Esposito
A1 Colin Farrell
A1 David Feng
A1 Olivia Fong
A1 Rohan Gala
A1 Clare Gamlin
A1 Amanda Gary
A1 Alexandra Glandon
A1 Jeff Goldy
A1 Melissa Gorham
A1 Lucas Graybuck
A1 Hong Gu
A1 Kristen Hadley
A1 Michael J. Hawrylycz
A1 Alex M. Henry
A1 DiJon Hill
A1 Madie Hupp
A1 Sara Kebede
A1 Tae Kyung Kim
A1 Lisa Kim
A1 Matthew Kroll
A1 Changkyu Lee
A1 Katherine E. Link
A1 Matthew Mallory
A1 Rusty Mann
A1 Michelle Maxwell
A1 Medea McGraw
A1 Delissa McMillen
A1 Alice Mukora
A1 Lindsay Ng
A1 Lydia Ng
A1 Kiet Ngo
A1 Philip R. Nicovich
A1 Aaron Oldre
A1 Daniel Park
A1 Hanchuan Peng
A1 Osnat Penn
A1 Thanh Pham
A1 Alice Pom
A1 Lydia Potekhina
A1 Ramkumar Rajanbabu
A1 Shea Ransford
A1 David Reid
A1 Christine Rimorin
A1 Miranda Robertson
A1 Kara Ronellenfitch
A1 Augustin Ruiz
A1 David Sandman
A1 Kimberly Smith
A1 Josef Sulc
A1 Susan M. Sunkin
A1 Aaron Szafer
A1 Michael Tieu
A1 Amy Torkelson
A1 Jessica Trinh
A1 Herman Tung
A1 Wayne Wakeman
A1 Katelyn Ward
A1 Grace Williams
A1 Zhi Zhou
A1 Jonathan Ting
A1 Uygar Sumbul
A1 Ed Lein
A1 Christof Koch
A1 Zizhen Yao
A1 Bosiljka Tasic
A1 Jim Berg
A1 Gabe J. Murphy
A1 Hongkui Zeng
YR 2020
UL http://biorxiv.org/content/early/2020/02/04/2020.02.03.932244.abstract
AB Neurons are frequently classified into distinct groups or cell types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 3,700 GABAergic mouse visual cortical neurons and reconstructed the local morphologies of 350 of those neurons. We found that most transcriptomic types (t-types) occupy specific laminar positions within mouse visual cortex, and many of those t-types exhibit consistent electrophysiological and morphological features. We observed that these properties could vary continuously between t-types, which limited the ability to predict specific t-types from other data modalities. Despite that, the data support the presence of at least 20 interneuron met-types that have congruent morphological, electrophysiological, and transcriptomic properties.HighlightsPatch-seq data obtained from >3,700 GABAergic cortical interneuronsComprehensive characterization of morpho-electric features of transcriptomic types20 interneuron met-types that have congruent properties across data modalitiesDifferent Sst met-types preferentially innervate different cortical layers