RT Journal Article
SR Electronic
T1 Super-Beacons: open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.20.912311
DO 10.1101/2020.01.20.912311
A1 Pereira, Pedro M.
A1 Gustafsson, Nils
A1 Marsh, Mark
A1 Mhlanga, Musa M.
A1 Henriques, Ricardo
YR 2020
UL http://biorxiv.org/content/early/2020/02/04/2020.01.20.912311.abstract
AB Localization based super-resolution microscopy relies on the detection of individual molecules cycling between fluorescent and non-fluorescent states. These transitions are commonly regulated by high-intensity illumination, imposing constrains to imaging hardware and producing sample photodamage. Here, we propose single-molecule self-quenching as a mechanism to generate spontaneous photoswitching independent of illumination. To demonstrate this principle, we developed a new class of DNA-based open-source Super-Resolution probes named Super-Beacons, with photoswitching kinetics that can be tuned structurally, thermally and chemically. The potential of these probes for live-cell friendly Super-Resolution Microscopy without high-illumination or toxic imaging buffers is revealed by imaging Interferon Inducible Transmembrane proteins (IFITMs) at sub-100nm resolutions.