RT Journal Article SR Electronic T1 Multi-organ transcriptomic landscape of Ambystoma velasci metamorphosis JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.02.06.937896 DO 10.1101/2020.02.06.937896 A1 Palacios-Martínez Janet A1 Caballero-Pérez Juan A1 Espinal-Centeno Annie A1 Marquez-Chavoya Gilberto A1 Lomelí Hilda A1 Salas-Vidal Enrique A1 Schnabel Denhi A1 Chimal-Monroy Jesus A1 Cruz-Ramírez Alfredo YR 2020 UL http://biorxiv.org/content/early/2020/02/07/2020.02.06.937896.abstract AB Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. These organisms experience major changes in their circulatory, respiratory, digestive, excretory and reproductive systems. We performed a transcriptional global analysis of heart, lung and gills during diverse stages of Ambystoma velasci metamorphosis. In our analyses, we identified eight gene clusters for each organ, according to the expression patterns of differentially expressed genes. We found 4,064 differentially expressed genes in the heart, 4,107 in the lung and 8,265 in the gills. Among the differentially expressed genes in the heart, we observed genes involved in the differentiation of cardiomyocytes in the interatrial zone, vasculogenesis and in the maturation of coronary vessels. In the lung, we found genes differentially expressed related to angiogenesis, alveolarization and synthesis of the surfactant protein. In the case of the gills, the most prominent biological processes identified are degradation of extracellular matrix, apoptosis and keratin production. Our study sheds light on the transcriptional responses and the pathways involved in the transformation of the facultative metamorphic salamander A. velasci in an organ-specific manner.