RT Journal Article SR Electronic T1 DNA Content and Ploidy Estimation of Festuca ovina Accessions by Flow Cytometry JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.02.06.938100 DO 10.1101/2020.02.06.938100 A1 Qiu, Yinjie A1 Hamernick, Sierra A1 Ortiz, Joan Barreto A1 Watkins, Eric YR 2020 UL http://biorxiv.org/content/early/2020/02/07/2020.02.06.938100.1.abstract AB Festuca ovina is a fine fescue that is used as a low-input turfgrass. The ploidy levels of F. ovina accessions held by the USDA National Plant Germplasm System (NPGS) are unknown, limiting the use of the germplasm in breeding programs. The objective of this study was to determine DNA content and estimate ploidy of these 127 accessions. Among the accessions, we identified a wide range of ploidy levels from diploid to octoploid. We also found the accessions with higher ploidy levels usually had larger seed size. These results will be informative to plant breeders and researchers using germplasm from the F. ovina collection and point to challenges in maintaining polyploid, outcrossing germplasm seed stocks in common nurseries.