PT  - JOURNAL ARTICLE
AU  - Philip M. Borden
AU  - Peng Zhang
AU  - Amol V. Shivange
AU  - Jonathan S. Marvin
AU  - Joseph Cichon
AU  - Chuntao Dan
AU  - Kaspar Podgorski
AU  - Antonio Figueiredo
AU  - Ondrej Novak
AU  - Masashi Tanimoto
AU  - Eiji Shigetomi
AU  - Mark A. Lobas
AU  - Hyuntae Kim
AU  - Paula K. Zhu
AU  - Yajun Zhang
AU  - W. Sharon Zheng
AU  - ChengCheng Fan
AU  - Guangfu Wang
AU  - Bowen Xiang
AU  - Li Gan
AU  - Guang-Xian Zhang
AU  - Kaiming Guo
AU  - Li Lin
AU  - Yuan Cai
AU  - Andrew G. Yee
AU  - Abhi Aggarwal
AU  - Christopher P. Ford
AU  - Douglas C. Rees
AU  - Dirk Dietrich
AU  - Baljit S. Khakh
AU  - Jeremy S. Dittman
AU  - Wen-Biao Gan
AU  - Minoru Koyama
AU  - Vivek Jayaraman
AU  - Joseph F. Cheer
AU  - Henry A. Lester
AU  - J. Julius Zhu
AU  - Loren L. Looger
TI  - A fast genetically encoded fluorescent sensor for faithful <em>in vivo</em> acetylcholine detection in mice, fish, worms and flies
AID  - 10.1101/2020.02.07.939504
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.07.939504
4099  - http://biorxiv.org/content/early/2020/02/08/2020.02.07.939504.short
4100  - http://biorxiv.org/content/early/2020/02/08/2020.02.07.939504.full
AB  - Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all animals.