TY - JOUR T1 - Axonal mechanisms mediating GABA-A receptor inhibition of striatal dopamine release JF - bioRxiv DO - 10.1101/2020.02.09.941179 SP - 2020.02.09.941179 AU - Paul F. Kramer AU - Emily L. Twedell AU - Jung Hoon Shin AU - Renshu Zhang AU - Zayd M. Khaliq Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/02/11/2020.02.09.941179.abstract N2 - Axons of midbrain dopaminergic neurons innervate the striatum where they contribute to movement and reinforcement learning. Past work has shown that striatal GABA tonically inhibits dopamine release, but whether GABA-A receptors directly modulate transmission or act indirectly through circuit elements is unresolved. Here, we use whole-cell and perforated-patch recordings to test for GABA-A receptors on the main dopaminergic neuron axons and branching processes within striatum. Application of GABA depolarized axons, but also decreased the amplitude of axonal spikes, limited propagation and reduced striatal dopamine release. The mechanism of inhibition involved sodium channel inactivation and shunting. Lastly, we show that the positive allosteric modulator diazepam enhanced GABA-A currents on dopaminergic neuron axons and directly inhibited release, but also likely acts by reducing excitatory drive from cholinergic interneurons. Thus, we reveal the mechanisms of GABA-A receptor modulation of dopamine release and provide new insight into the actions of benzodiazepines within the striatum. ER -