PT  - JOURNAL ARTICLE
AU  - Miysaa I. Abdelmageed
AU  - Abdelrahman H. Abdelmoneim
AU  - Mujahed I. Mustafa
AU  - Nafisa M. Elfadol
AU  - Naseem S. Murshed
AU  - Shaza W. Shantier
AU  - Abdelrafie M. Makhawi
TI  - Design of multi epitope-based peptide vaccine against E protein of human 2019-nCoV: An immunoinformatics approach
AID  - 10.1101/2020.02.04.934232
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.04.934232
4099  - http://biorxiv.org/content/early/2020/02/11/2020.02.04.934232.short
4100  - http://biorxiv.org/content/early/2020/02/11/2020.02.04.934232.full
AB  - Background on the late December 2019, a new endemic has been spread across Wuhan City, China; within a few weeks, a novel coronavirus designated as 2019 novel coronavirus (2019-nCoV) was announced by the World Health Organization (WHO). In late January 2020, WHO declared the outbreak a “public-health emergency of international concern” due to the rapid spreading and increasing worldwide. There is no vaccine or approved treatment for this emerging infection; therefore, the objective of this paper is to design a multi epitope peptide vaccine against 2019-nCoV using immunoinformatics approach.Method We will highlight a technique facilitating the combination of immunoinformatics approach with comparative genomic approach to determine our potential target for designing the T cell epitopes-based peptide vaccine using the envelope protein of 2019-nCoV as a target.Results Extensive mutations, insertion and deletion were discovered with comparative sequencing in 2019-nCoV strain; in addition, 10 MHC1 and MHC2 related peptides were promising candidates for vaccine design with adequate world population coverage of 88.5% and 99.99% respectively.Conclusion T cell epitopes-based peptide vaccine was designed for 2019-nCoV using envelope protein as an immunogenic target; nevertheless, the proposed T cell epitopes-based peptide vaccine rapidly needs to validates clinically to ensure its safety and immunogenic profile to assist on stopping this epidemic before it leads to devastating global outbreaks.