RT Journal Article
SR Electronic
T1 Computer simulation of knotted proteins unfold and translocation through nano-pores
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 378901
DO 10.1101/378901
A1 Shahzad, M. A.
YR 2018
UL http://biorxiv.org/content/early/2018/07/27/378901.abstract
AB We study the unfold and translocation of knotted protein, YibK and YbeA, through α-hemolysin nano-pore via a coarse grained computational model. We observe that knot of protein unfold in advance before the translocation take place. We also characterized the translocation mechanism by studying the thermodynamical and kinetic properties of the process. In particular, we study the average of translocation time, and the translocation probability as a function of pulling force F acting in the channel. In limit of low pulling inward constant force acting along the axis of the pore, the YibK knotted protein takes longer average translocation time as compare to YbeA knotted protein.