RT Journal Article
SR Electronic
T1 Pou5f3 and Sox19b select gene expression repertoire at Zygotic Genome Activation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.16.949362
DO 10.1101/2020.02.16.949362
A1 Meijiang Gao
A1 Marina Veil
A1 Marcus Rosenblatt
A1 Anna Gebhard
A1 Helge Hass
A1 Lenka Buryanova
A1 Lev Y. Yampolsky
A1 Björn Grüning
A1 Jens Timmer
A1 Daria Onichtchouk
YR 2020
UL http://biorxiv.org/content/early/2020/02/16/2020.02.16.949362.abstract
AB PouV and SoxB1 family transcription factors (TFs) have emerged as master regulators of cell fate transitions. To investigate the genetic interactions between Pou5f3 and Sox19b in zebrafish embryos passing through Zygotic Genome Activation (ZGA), we combined time-resolved mutant transcription analysis using the novel tool RNA-sense, chromatin state and phenotypic assays. We distinguish four types of embryonic enhancers, differentially regulated by the two TFs. Pou5f3 is critical for activation of enhancer types 1 and 2, which are responsible for transcription of genes involved in gastrulation and ventral genes. Pou5f3 or Sox19b prevent premature activation of type 3 and 4 enhancers, which are responsible for transcription of organogenesis regulators, differentiation factors and dorsal genes. We also show that the balance between Sox19b and Pou5f3 is important for bulk ZGA timing. Our results uncover how independent activities of maternal Pou5f3 and Sox19b add up or antagonize to determine the early gene expression repertoire.Bullet pointsPou5f3 and Sox19b bind independently to DNADisbalance between maternal Pou5f3 and Sox19b delays ZGAPou5f3 suppresses premature transcription of neural patterning genes, activated by SoxB1 factorsPou5f3 and Sox19b synergistically suppress premature transcription of a wide range of differentiation genesPou5f3 and Sox19b restrict the dorsal organizer