PT  - JOURNAL ARTICLE
AU  - Ian D. Peikon
AU  - Diana I. Gizatullina
AU  - Anthony M. Zador
TI  - <em>In vivo</em> generation of DNA sequence diversity for cellular barcoding
AID  - 10.1101/004499
DP  - 2014 Jan 01
TA  - bioRxiv
PG  - 004499
4099  - http://biorxiv.org/content/early/2014/04/24/004499.short
4100  - http://biorxiv.org/content/early/2014/04/24/004499.full
AB  - Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic “barcode” that can be recovered by DNA sequencing. We demonstrate the feasibility of this technique in bacterial cells. This method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.