PT  - JOURNAL ARTICLE
AU  - Ying Feng
AU  - Yao Wang
AU  - Chen Yang
AU  - Ardalan Naseri
AU  - Thoru Pederson
AU  - Jing Zheng
AU  - Xiao Xiao
AU  - Shaojie Zhang
AU  - Wei Xie
AU  - Hanhui Ma
TI  - CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
AID  - 10.1101/2020.02.18.954610
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.18.954610
4099  - http://biorxiv.org/content/early/2020/02/19/2020.02.18.954610.short
4100  - http://biorxiv.org/content/early/2020/02/19/2020.02.18.954610.full
AB  - Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1α condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1α condensates possibly driven by phase separation.