RT Journal Article
SR Electronic
T1 Improved daylight vision following AAV-mediated expression of <em>R9AP</em> in murine rod photoreceptors
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.19.955765
DO 10.1101/2020.02.19.955765
A1 Nishiguchi, Koji M.
A1 Fujita, Kosuke
A1 Cristante, Enrico
A1 Bainbridge, James W.
A1 Douglas, Ronald H.
A1 Nakazawa, Toru
A1 Smith, Alexander J.
A1 Ali, Robin R.
YR 2020
UL http://biorxiv.org/content/early/2020/02/20/2020.02.19.955765.abstract
AB Cone photoreceptors mediate daylight vision and are the primary cells responsible for vision in humans. Cone dysfunction leads to poor quality daylight vision because rod photoreceptors become saturated and non-functional at high light levels. Here we demonstrate that in mice lacking cone function, AAV-mediated over-expression of Rgs9-anchor protein (R9AP), a critical component of the GTPase complex that mediates the deactivation of the phototransduction cascade, results in desensitization of rod function and a “photopic shift” of the rod-driven electroretinogram. This treatment enables rods to respond to brighter light (up to ∼2.0 log) with increased visually-evoked cortical responses to high intensity stimulation. These results suggest that AAV-mediated transfer of R9ap into rods might be used to improve daylight vision in humans visually handicapped by cone dysfunction.