PT - JOURNAL ARTICLE AU - The Tabula Muris Consortium AU - Angela Oliveira Pisco AU - Aaron McGeever AU - Nicholas Schaum AU - Jim Karkanias AU - Norma F Neff AU - Spyros Darmanis AU - Tony Wyss-Coray AU - Stephen R Quake TI - A Single Cell Transcriptomic Atlas Characterizes Aging Tissues in the Mouse AID - 10.1101/661728 DP - 2020 Jan 01 TA - bioRxiv PG - 661728 4099 - http://biorxiv.org/content/early/2020/02/21/661728.short 4100 - http://biorxiv.org/content/early/2020/02/21/661728.full AB - Aging is characterized by a progressive loss of physiological integrity, leading to impaired function and increased vulnerability to death1. Despite rapid advances over recent years, many of the molecular and cellular processes which underlie progressive loss of healthy physiology are poorly understood2. To gain a better insight into these processes we have created a single cell transcriptomic atlas across the life span of Mus musculus which includes data from 23 tissues and organs. We discovered cell-specific changes occurring across multiple cell types and organs, as well as age related changes in the cellular composition of different organs. Using single-cell transcriptomic data we were able to assess cell type specific manifestations of different hallmarks of aging, such as senescence3, genomic instability4 and changes in the organism’s immune system2. This Tabula Muris Senis provides a wealth of new molecular information about how the most significant hallmarks of aging are reflected in a broad range of tissues and cell types.