PT  - JOURNAL ARTICLE
AU  - Andrew K Lawton
AU  - Tyler Engstrom
AU  - Daniel Rohrbach
AU  - Masaaki Omura
AU  - Daniel H Turnbull
AU  - Jonathan Mamou
AU  - Teng Zhang
AU  - J. M. Schwarz
AU  - Alexandra L Joyner
TI  - Brain folding is initiated by mechanical constraints without a cellular pre-pattern
AID  - 10.1101/382887
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 382887
4099  - http://biorxiv.org/content/early/2018/08/01/382887.short
4100  - http://biorxiv.org/content/early/2018/08/01/382887.full
AB  - During human brain development the cerebellum and cerebral cortex fold into robust patterns that increase and compartmentalize neural circuits. Although differential expansion of elastic materials has been proposed to explain brain folding, the cellular and physical processes responsible at the time of folding have not been defined. Here we used the murine cerebellum, with 8-10 folds, as a tractable model to study brain folding. At folding initiation we considered the cerebellum as a bilayer system with a fluidlike outer layer of proliferating precursors and an incompressible core. We discovered that there is no obvious cellular pre-pattern for folding, since when folding initiates, the precursors within the outer layer have uniform sizes, shapes and proliferation, as well as a distribution of glial fibers. Furthermore, although differential expansion is created by the outer layer expanding faster than the core at folding initiation, thickness variations arise in the outer layer that are inconsistent with elastic material models. A multiphase model was applied that includes radial and circumferential tension and mechanical constraints derived from in vivo measurements. Our results demonstrate that cerebellar folding emerges from mechanical forces generated by uniform cell behaviors. We discuss how our findings apply to human cerebral cortex folding.