RT Journal Article SR Electronic T1 A non-canonical Hippo pathway regulates spindle disassembly and cytokinesis during meiosis in Saccharomyces cerevisiae JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.02.21.959619 DO 10.1101/2020.02.21.959619 A1 Scott M. Paulissen A1 Cindy A. Hunt A1 Christian J. Slubowski A1 Yao Yu A1 Dang Truong A1 Xheni Mucelli A1 Hung T. Nguyen A1 Shayla Newman-Toledo A1 Aaron M. Neiman A1 Linda S. Huang YR 2020 UL http://biorxiv.org/content/early/2020/02/22/2020.02.21.959619.abstract AB Meiosis in the budding yeast Saccharomyces cerevisiae is used to create haploid yeast spores from a diploid mother cell. During meiosis II, cytokinesis occurs by closure of the prospore membrane, a membrane that initiates at the spindle pole body and grows to surround each of the haploid meiotic products. Timely prospore membrane closure requires SPS1, which encodes a STE20-family GCKIII kinase. To identify genes that may activate SPS1, we utilized a histone phosphorylation defect of sps1 mutants to screen for genes with a similar phenotype and found that cdc15 shared this phenotype. CDC15 encodes a Hippo-like kinase that is part of the mitotic exit network. We find that Sps1 complexes with Cdc15, that Sps1 phosphorylation requires Cdc15, and that CDC15 is also required for timely prospore membrane closure. We also find that SPS1, like CDC15, is required for meiosis II spindle disassembly and sustained anaphase II release of Cdc14 in meiosis. However, the NDR-kinase complex encoded by DBF2/DBF20 MOB1 which functions downstream of CDC15 in mitotic cells, does not appear to play a role in spindle disassembly, timely prospore membrane closure, or sustained anaphase II Cdc14 release. Taken together, our results suggest that the mitotic exit network is rewired for exit from meiosis II, such that SPS1 replaces the NDR-kinase complex downstream of CDC15.