RT Journal Article
SR Electronic
T1 Cytoplasmic sharing through apical membrane remodeling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.22.960187
DO 10.1101/2020.02.22.960187
A1 Nora G. Peterson
A1 Benjamin M. Stormo
A1 Kevin P. Schoenfelder
A1 Juliet S. King
A1 Rayson R. S. Lee
A1 Donald T. Fox
YR 2020
UL http://biorxiv.org/content/early/2020/02/22/2020.02.22.960187.abstract
AB Multiple nuclei sharing a common cytoplasm are found in diverse tissues, organisms, and diseases. Yet, multinucleation remains a poorly understood biological property. Cytoplasm sharing invariably involves plasma membrane breaches. In contrast, we discovered cytoplasm sharing without membrane breaching in highly resorptive Drosophila rectal papillae. During a six-hour developmental window, 100 individual papillar cells assemble a multinucleate cytoplasm, allowing passage of proteins of at least 27kDa throughout papillar tissue. Papillar cytoplasm sharing does not employ canonical mechanisms such as failed cytokinesis or muscle fusion pore regulators. Instead, sharing requires gap junction proteins (normally associated with transport of molecules <1kDa), which are positioned by membrane remodeling GTPases. Our work reveals a new role for apical membrane remodeling in converting a multicellular epithelium into a giant multinucleate cytoplasm.ONE SENTENCE SUMMARY Apical membrane remodeling in a resorptive Drosophila epithelium generates a shared multinuclear cytoplasm.