TY - JOUR T1 - Antinociceptive modulation by the adhesion-GPCR CIRL promotes mechanosensory signal discrimination JF - bioRxiv DO - 10.1101/2020.02.24.963439 SP - 2020.02.24.963439 AU - Sven Dannhäuser AU - Thomas J. Lux AU - Chun Hu AU - Mareike Selcho AU - Jeremy Tsung-Chieh Chen AU - Nadine Ehmann AU - Divya Sachidanandan AU - Matthias Pawlak AU - Tobias Langenhan AU - Peter Soba AU - Heike Rittner AU - Robert J. Kittel Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/02/25/2020.02.24.963439.abstract N2 - Adhesion-type G protein-coupled receptors (aGPCRs) participate in a vast range of physiological processes. Correspondingly, these receptors are associated with diverse human diseases, such as developmental disorders, defects of the nervous system, allergies and cancer. Several aGPCRs have recently been linked to mechanosensitive functions suggesting that processing of mechanical stimuli may be a common feature of this receptor family. CIRL (ADGRL/Latrophilin, LPHN), one of the oldest members of the aGPCR family, sensitizes sensory responses of larval Drosophila to gentle touch and sound by amplifying mechanosensory signal transduction in low-threshold mechanoreceptors (Scholz et al., 2015; 2017). In the present study, we show that Cirl is also expressed in high-threshold mechanical nociceptors where it adjusts nocifensive behaviour under physiological and pathophysiological conditions. Optogenetic in vivo experiments indicate that CIRL quenches cAMP levels in both mechanosensory submodalities. However, contrasting its effect in touch sensitive neurons, CIRL dampens the response of nociceptors to mechanical stimulation. Consistent with this finding, rat nociceptors display a drop in Cirl1 expression during allodynia. Taken together, these results demonstrate that CIRL exerts opposing modulatory functions in low-threshold mechanosensors and high-threshold nociceptors. This intriguing bipolar action likely facilitates the separation of mechanosensory signals carrying different physiological information. ER -