PT  - JOURNAL ARTICLE
AU  - McRaven, Christopher
AU  - Tanese, Dimitrii
AU  - Zhang, Lixia
AU  - Yang, Chao-Tsung
AU  - Ahrens, Misha B.
AU  - Emiliani, Valentina
AU  - Koyama, Minoru
TI  - High-throughput cellular-resolution synaptic connectivity mapping <em>in vivo</em> with concurrent two-photon optogenetics and volumetric Ca<sup>2+</sup> imaging
AID  - 10.1101/2020.02.21.959650
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.21.959650
4099  - http://biorxiv.org/content/early/2020/02/25/2020.02.21.959650.short
4100  - http://biorxiv.org/content/early/2020/02/25/2020.02.21.959650.full
AB  - The ability to measure synaptic connectivity and properties is essential for understanding neuronal circuits. However, existing methods that allow such measurements at cellular resolution are laborious and technically demanding. Here, we describe a system that allows such measurements in a high-throughput way by combining two-photon optogenetics and volumetric Ca2+ imaging with whole-cell recording. We reveal a circuit motif for generating fast undulatory locomotion in zebrafish.