TY - JOUR T1 - Whole brain optoacoustic tomography reveals strain-specific regional beta-amyloid densities in Alzheimer’s disease amyloidosis models JF - bioRxiv DO - 10.1101/2020.02.25.964064 SP - 2020.02.25.964064 AU - Ruiqing Ni AU - Xose Luis Dean-Ben AU - Daniel Kirschenbaum AU - Markus Rudin AU - Zhenyue Chen AU - Alessandro Crimi AU - Fabian F. Voigt AU - K. Peter R. Nilsson AU - Fritjof Helmchen AU - Roger Nitsch AU - Adriano Aguzzi AU - Daniel Razansky AU - Jan Klohs Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/02/25/2020.02.25.964064.abstract N2 - Deposition of beta-amyloid (Aβ) deposits is one major histopathological hallmark of Alzheimer’s disease (AD). Here, we introduce volumetric multi-spectral optoacoustic tomography (vMSOT), which covers 10×10×10 mm3 field-of-view, capable of 3D whole mouse brain imaging. We show for the first time the optoacoustic properties of oxazine-derivative AOI987 probe, which binds to Aβ, and the application of vMSOT for the quantification of brain-wide Aβ deposition. Administration of AOI987 to two common transgenic mouse strains of AD amyloidosis led to a retention of the probe in Aβ-laden brain regions. Co-registered of vMSOT data to a brain atlas revealed strain-specific pattern of AOI987 uptake. A comparison with ex vivo light-sheet microscopy in cleared mouse brains showed a good correspondence in Aβ distribution. Lastly, we demonstrate the specificity of the AOI987 probe by immunohistochemistry. vMSOT with AOI987 facilitates preclinical brain region-specific studies of Aβ spread and accumulation, and the monitoring of putative treatments targeting Aβ. ER -