PT  - JOURNAL ARTICLE
AU  - Peter F. Cowman
AU  - Andrea M. Quattrini
AU  - Thomas C.L. Bridge
AU  - Gregory J. Watkins-Colwell
AU  - Nur Fadli
AU  - Mila Grinblat
AU  - Thomas E. Roberts
AU  - Catherine S. McFadden
AU  - David J. Miller
AU  - Andrew H. Baird
TI  - An enhanced target-enrichment bait set for Hexacorallia provides phylogenomic resolution of the staghorn corals (Acroporidae) and close relatives
AID  - 10.1101/2020.02.25.965517
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.25.965517
4099  - http://biorxiv.org/content/early/2020/02/26/2020.02.25.965517.short
4100  - http://biorxiv.org/content/early/2020/02/26/2020.02.25.965517.full
AB  - The phylogenetic utility of targeted enrichment methods has been demonstrated in taxa that often have a history of single gene marker development. These genomic capture methods are now being applied to resolve evolutionary relationships from deep to shallow timescales in clades that were previously deficient in molecular marker development and lacking robust morphological characters that reflect evolutionary relationships. Effectively capturing 1000s of loci, however, in a diverse group across a broad time scale requires a bait set that incorporates multiple baits per locus. We redesigned a custom bait set for the cnidarian class Anthozoa to target 1,436 UCE loci and 1,572 exon regions within the subclass Hexacorallia. We test this redesigned bait set on 99 specimens of hard corals (Scleractinia) spanning both the “complex” (Acroporidae, Agariciidae) and “robust” (Fungiidae) clades. With focused sampling in the staghorn coral genus Acropora we explore the ability of capture data to inform the taxonomy of a clade deficient in molecular resolution. A mean of 1850 (± 298) loci were captured per taxon (955 UCEs, 894 exons). A 75% complete concatenated alignment included 1792 loci (991 UCE, 801 exons) and ∼1.87 million base pairs. Parsimony informative sites varied from 48% for alignments including all three families, to 1.5% among samples within a single Acropora species. Maximum likelihood and Bayesian analyses recover highly resolved topologies and robust molecular relationships not previously found with traditional markers within the Acroporidae. Species level relationships within the Acropora genus do not support traditional morphological groups or morphological phylogenies. Both UCE and exon datasets delineated six well-supported clades within Acropora. The enhanced bait set for Hexacorallia will allow researchers to survey the evolutionary history of important groups of reef building corals where previous molecular marker development has been unsuccessful.