TY - JOUR T1 - CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering JF - bioRxiv DO - 10.1101/2020.02.26.965889 SP - 2020.02.26.965889 AU - DP. Anderson AU - HJ. Benns AU - EW. Tate AU - MA Child Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/02/26/2020.02.26.965889.abstract N2 - Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time consuming post-hoc curation, simplifying gRNA outputs, and reducing CPU times. ER -