RT Journal Article SR Electronic T1 Mice developing mammary tumors evolve T cell sequences shared with human breast cancer patients JF bioRxiv FD Cold Spring Harbor Laboratory SP 371260 DO 10.1101/371260 A1 Miri Gordin A1 Hagit Philip A1 Alona Zilberberg A1 Moriah Gidoni A1 Raanan Margalit A1 Christopher Clouser A1 Kristofor Adams A1 Francois Vigneault A1 Irun R. Cohen A1 Gur Yaari A1 Sol Efroni YR 2018 UL http://biorxiv.org/content/early/2018/08/06/371260.abstract AB Cancer immunotherapy by checkpoint blockade proves that an effective immune response to a tumor can be induced clinically. However, little is known about the evolution of tumor-associated T-cell receptor (TCR) repertoires without intervention. Here we studied TCR repertoire evolution in mice spontaneously developing mammary tumors; we sequenced peripheral blood alpha and beta TCRs of CD4+CD62L+CD44− T cells monthly for 8 months in 10 FVB/NJ mice transgenic at the Erbb2 locus, all developing tumors; 5 FVB/NJ mice without the transgene were age-matched controls. Sequences were either private (restricted to one mouse) or public (shared among mice); public sequences were either exclusive to the tumor group or inclusive among different groups. We now report that 1), public AA sequences were each encoded by many different nucleotide sequences (NT) recombinations (convergent recombination; CR); 2) mice developing tumors evolved tumor-exclusive public sequences, derived initially from private or from inclusive public sequences; and 3) tumor-exclusive public sequences in mice were also present among published public TCR sequences from human breast cancer patients. These cross-species tumor-exclusive TCR sequences manifested high CR; but the AA sequences shared by mice and humans did not share NT sequences. Thus, tumor-exclusive TCR AA sequences across species are selected from different NT recombination events. The roles of tumor-exclusive TCR repertoires in advancing or inhibiting tumor development and the effects of tumor immunotherapy on these T cells remain to be seen.