PT  - JOURNAL ARTICLE
AU  - Nivedya Swarnalekha
AU  - David Schreiner
AU  - Ludivine C Litzler
AU  - Saadia Iftikhar
AU  - Daniel Kirchmeier
AU  - Marco Künzli
AU  - Carolyn G King
TI  - Redefining CD4 T cell residency: Helper T cells orchestrate protective humoral immunity in the lung
AID  - 10.1101/2020.02.28.963280
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.28.963280
4099  - http://biorxiv.org/content/early/2020/02/28/2020.02.28.963280.short
4100  - http://biorxiv.org/content/early/2020/02/28/2020.02.28.963280.full
AB  - Influenza is a severe and acute respiratory pathogen, and a significant cause for morbidity, particularly in young children and the elderly. Following influenza infection, clonally expanded T cells take up permanent residence in the lung where they are poised to rapidly respond to challenge infection. The non-circulating status of these tissue resident memory (TRM) cells makes them an attractive target for vaccination. While many studies have characterized CD8 TRM cells, less is known about the heterogeneity and protective capacity of CD4 TRM cells. Here we characterized the dynamics and transcriptional regulation of lung resident CD4 T cells to define a non-lymphoid signature that removes the bias created by the prevalence of Th1 helper cells during viral infection. We identified a novel population of long-lived T resident helper (TRH) cells that requires intrinsic Bcl6 expression for their differentiation. Although TRH cells also depend on B cells, they are generated independently of T follicular helper effector cells in the lymph node. In contrast to lung resident Th1 cells, TRH cells are tightly co-localized with B cells in inducible Bronchus Associated Lymphoid Tissue (iBALT). Deletion of Bcl6 in CD4 T cells prior to heterotypic challenge infection results in redistribution of CD4 T cells outside of iBALT areas and impaired local antibody production. These data highlight lung iBALT as a niche for the homeostasis and survival of TRH cells, and further suggest that vaccination strategies to selectively induce TRH cells can improve protective immunity in the tissue.