RT Journal Article
SR Electronic
T1 <em>In Vivo</em> Analysis of RNA Proximity Proteomes Using RiboPro
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.28.970442
DO 10.1101/2020.02.28.970442
A1 Xianzhi Lin
A1 Kate Lawrenson
YR 2020
UL http://biorxiv.org/content/early/2020/02/28/2020.02.28.970442.abstract
AB Functional and mechanistic annotation of uncharacterized long noncoding RNAs is challenging and often requires identification of interacting proteins. Here we report RiboPro, a flexible method that leverages the RNA-binding specificity of inactive Cas13b and proximity labeling activity of peroxidase APEX to permit rapid and unbiased discovery of target RNA binding proteins in vivo. RiboPro of poly(A)+ RNA reveals insights into poly (A)+ RNA nucleocytoplasmic transport, localization, turnover, and higher-order structure.