RT Journal Article
SR Electronic
T1 Altered B cells homeostasis in child-onset immunoglobulin A vasculitis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.28.969444
DO 10.1101/2020.02.28.969444
A1 Deying Liu
A1 Yanfang Jiang
A1 Jinghua Wang
A1 Jinxiang Liu
A1 Meng Xu
A1 Congcong Liu
A1 Sirui Yang
YR 2020
UL http://biorxiv.org/content/early/2020/02/28/2020.02.28.969444.abstract
AB Background Immunoglobulin A vasculitis (IgAV), also called Henoch–Schönlein purpura, is a systemic small vessels vasculitis with immunoglobulin A1-dominant immune deposits. B-cells are a heterogeneous population with unique subsets distinguished by their phenotypes and cytokine production. Here, we explored the status of B cell subsets in patients with IgAV.Methods Thirty IgAV patients and fifteen age- and sex-matched healthy individuals were enrolled in this study. Fresh blood samples were collected from both healthy and IgAV patients. Upon the distinct expressions of CD3, CD19, CD20, CD38, CD27 and IgD, peripheral blood mononuclear cells (PBMCs) were initially categorized into plasmablasts and memory B cells. Subsequently, using surface markers including CD138 and IgM, and intracellular markers containing IgM and IgG, plasmablasts and memory B cells were further divided into distinct subgroups. A total of eleven populations were detected using multiple flow cytometry.Results CD3-CD19+IgD+CD27-, CD3-CD19+CD20-CD38+, CD3-CD19+CD20-CD38+IgM+, and CD3-CD19+CD20-CD38+CD138+ B cells were larger in patients with IgAV than in the HCs. Only CD3-CD19+IgD-CD27+IgM+ B cell counts were reduced in IgAV. The elevated B cell numbers returned to normal after treatment. Plasma and plasmablast B cell numbers correlated with plasma IgA levels. On the contrary, CD3-CD19+IgD-CD27+IgM+ B cell numbers were negatively proportional to the plasma IgA levels while naïve B cell numbers correlated with plasma and plasmablast B cell counts.Conclusions We hypothesized that immunoglobulin production was abnormally elevated in IgAV and could be explained by altered B-cell subset homeostasis.IgAVImmunoglobulin A vasculitisGCgerminal centerHChealthy controlPBMCPeripheral blood mononuclear cellBCRB-cell receptor