TY - JOUR T1 - Transcriptome profiling of the branchial arches reveals cell type composition and a conserved signature of neural crest cell invasion JF - bioRxiv DO - 10.1101/2020.02.28.969915 SP - 2020.02.28.969915 AU - Jason A Morrison AU - Rebecca McLennan AU - Jessica M Teddy AU - Allison R Scott AU - Jennifer C Kasemeier-Kulesa AU - Madelaine M Gogol AU - Paul M Kulesa Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/02/28/2020.02.28.969915.abstract N2 - The vertebrate branchial arches that give rise to structures of the head, neck, and heart form with very dynamic tissue growth and well-choreographed neural crest, ectoderm, and mesoderm cell dynamics. Although this morphogenesis has been studied by marker expression and fate-mapping, the mechanisms that control the collective migration and diversity of the neural crest and surrounding tissues remain unclear, in part due to the effects of averaging and need for cell isolation in conventional transcriptome analysis experiments of multiple cell populations. We used label free single cell RNA sequencing on 95,000 individual cells at 2 developmental stages encompassing formation of the first four chick branchial arches to measure the transcriptional states that define the cellular hierarchy and invasion signature of the migrating neural crest. The results confirmed basic features of cell type diversity and led to the discovery of many novel markers that discriminate between axial level and distal-to-proximal cell populations within the branchial arches and neural crest streams. We identified the transcriptional signature of the most invasive neural crest that is conserved within each branchial arch stream and elucidated a set of genes common to other cell invasion signatures in types in cancer, wound healing and development. These data robustly delineate molecularly distinct cell types within the branchial arches and identify important molecular transitions within the migrating neural crest during development. ER -