RT Journal Article
SR Electronic
T1 Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 386110
DO 10.1101/386110
A1 Steinegger, Martin
A1 Mirdita, Milot
A1 Söding, Johannes
YR 2018
UL http://biorxiv.org/content/early/2018/08/07/386110.abstract
AB The open-source de-novo Protein-Level ASSembler Plass (https://plass.mmseqs.org) assembles six-frame-translated sequencing reads into protein sequences. It recovers 2 to 10 times more protein sequences from complex metagenomes and can assemble huge datasets. We assembled two redundancy-filtered reference protein catalogs, 2 billion sequences from 640 soil samples (SRC) and 292 million sequences from 775 marine eukaryotic metatranscriptomes (MERC), the largest free collections of protein sequences.