TY - JOUR T1 - The <em>cis</em>-regulatory codes of response to combined heat and drought stress in <em>Arabidopsis thaliana</em> JF - bioRxiv DO - 10.1101/2020.02.28.969261 SP - 2020.02.28.969261 AU - Christina B. Azodi AU - John P. Lloyd AU - Shin-Han Shiu Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/02/29/2020.02.28.969261.abstract N2 - Plants respond to their environment by dynamically modulating gene expression. A powerful approach for understanding how these responses are regulated is to integrate information about cis-regulatory elements (CREs) into models called cis-regulatory codes. Transcriptional response to combined stress is typically not the sum of the responses to the individual stresses. However, cis-regulatory codes underlying combined stress response have not been established. Here we modeled transcriptional response to single and combined heat and drought stress in Arabidopsis thaliana. We grouped genes by their pattern of response (independent, antagonistic, synergistic) and trained machine learning models to predict their response using putative CREs (pCREs) as features (median F-measure = 0.64). We then developed a deep learning approach to integrate additional omics information (sequence conservation, chromatin accessibility, histone modification) into our models, improving performance by 6.2%. While pCREs important for predicting independent and antagonistic responses tended to resemble binding motifs of transcription factors associated with heat and/or drought stress, important synergistic pCREs resembled binding motifs of transcription factors not known to be associated with stress. These findings demonstrate how in silico approaches can improve our understanding of the complex codes regulating response to combined stress and help us identify prime targets for future characterization. ER -