RT Journal Article SR Electronic T1 The cis-regulatory codes of response to combined heat and drought stress in Arabidopsis thaliana JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.02.28.969261 DO 10.1101/2020.02.28.969261 A1 Christina B. Azodi A1 John P. Lloyd A1 Shin-Han Shiu YR 2020 UL http://biorxiv.org/content/early/2020/02/29/2020.02.28.969261.abstract AB Plants respond to their environment by dynamically modulating gene expression. A powerful approach for understanding how these responses are regulated is to integrate information about cis-regulatory elements (CREs) into models called cis-regulatory codes. Transcriptional response to combined stress is typically not the sum of the responses to the individual stresses. However, cis-regulatory codes underlying combined stress response have not been established. Here we modeled transcriptional response to single and combined heat and drought stress in Arabidopsis thaliana. We grouped genes by their pattern of response (independent, antagonistic, synergistic) and trained machine learning models to predict their response using putative CREs (pCREs) as features (median F-measure = 0.64). We then developed a deep learning approach to integrate additional omics information (sequence conservation, chromatin accessibility, histone modification) into our models, improving performance by 6.2%. While pCREs important for predicting independent and antagonistic responses tended to resemble binding motifs of transcription factors associated with heat and/or drought stress, important synergistic pCREs resembled binding motifs of transcription factors not known to be associated with stress. These findings demonstrate how in silico approaches can improve our understanding of the complex codes regulating response to combined stress and help us identify prime targets for future characterization.