PT - JOURNAL ARTICLE AU - Tara D. Fischer AU - Pramod K. Dash AU - Jun Liu AU - M. Neal Waxham TI - Morphology of Mitochondria in Spatially Restricted Axons Revealed by Cryo-Electron Tomography AID - 10.1101/273052 DP - 2018 Jan 01 TA - bioRxiv PG - 273052 4099 - http://biorxiv.org/content/early/2018/08/08/273052.short 4100 - http://biorxiv.org/content/early/2018/08/08/273052.full AB - Neurons project axons to local and distal sites and can display heterogeneous morphologies with limited physical dimensions that may influence the structure of large organelles such as mitochondria. Using cryo-electron tomography (cryo-ET), we characterized native environments within axons and presynaptic varicosities to examine whether spatial restrictions within these compartments influence the morphology of mitochondria. Segmented tomographic reconstructions revealed distinctive morphological characteristics of mitochondria residing at the narrowed boundary between presynaptic varicosities and axons with limited physical dimensions (~80 nm), compared to mitochondria in non-spatially restricted environments. Furthermore, segmentation of the tomograms revealed discrete organizations between the inner and outer membranes, suggesting possible independent remodeling of each membrane in mitochondria at spatially restricted axonal/varicosity boundaries. Thus, cryo-ET of mitochondria within axonal subcompartments reveals that spatial restrictions do not obstruct mitochondria from residing within them but limited available space can influence their gross morphology and the organization of the inner and outer membranes. These findings offer new perspectives on the influence of physical and spatial characteristics of cellular environments on mitochondrial morphology and highlights the potential for remarkable structural plasticity of mitochondria to adapt to spatial restrictions within axons.