RT Journal Article
SR Electronic
T1 Structural basis of transcription-translation coupling and collision in bacteria
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.01.971028
DO 10.1101/2020.03.01.971028
A1 Michael William Webster
A1 Maria Takacs
A1 Chengjin Zhu
A1 Vita Vidmar
A1 Ayesha Eduljee
A1 Mo’men Abdelkareem
A1 Albert Weixlbaumer
YR 2020
UL http://biorxiv.org/content/early/2020/03/02/2020.03.01.971028.abstract
AB Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The pioneering ribosome potentially contacts RNA polymerase (RNAP), forming a supramolecular complex known as the expressome. The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here we present a series of structures representing uncoupled, coupled and collided expressome states determined by electron cryomicroscopy. A bridge between the ribosome and RNAP can be formed by the transcription factor NusG, stabilizing an otherwise variable interaction interface. Shortening of the intervening mRNA causes a substantial rearrangement that aligns the ribosome entrance-channel to the RNAP exit-channel. In this collided complex, NusG-linkage is no longer possible.These structures reveal mechanisms of coordination between transcription and translation and provide a framework for future study.One Sentence Summary Structures of the molecular assembly executing gene expression shed light on transcription translation coupling.